Reagent kit is intended for detection of Human papilloma virus (HPV) types 26 and 51 DNA in clinical specimens by real-time PCR. The kits contain reagents for real-time PCR only.

Introduction

“MOLgen DNA HPV 26/51 S1 Kit” is intended for the differential detection of DNA of human papillomavirus (HPV) types 26 and 51 DNA in clinical specimens (epithelial cells swabs) using the method of real-time polymerase chain reaction (PCR) with fluorescence detection of amplified product.

The extraction of DNA from clinical specimens can be performed using the “Molgen Universal Extraction Kit”.When using NA extraction kits of other manufacturers, it is highly recommended to use Internal Control sample (IC) manufactured by Adaltis S.r.l.

The results of PCR analysis are taken into account in complex diagnostics of disease.

Set 1 is intended for use with block-type PCR cyclers:AMPLIlab (Adaltis), iQ5 iCycler, CFX96 (Bio-Rad, USA), DT-96 (DNA-Technology, Russia).

Set 1 contains reagents requiredfor 96 tests, including control samples.

Principle Of Method

Real-time PCR is based on the detection of the fluorescence produced by a reporter molecule, which increases as the reaction proceeds. Reporter molecule is a dual-labeled DNA-probe that specifically binds to the target region of pathogens DNA. Fluorescence signal increases due to the separation of fluorescence dye and quencher by Taq DNA-polymerase exonuclease activity during amplification. PCR consists of repeated cycles: temperature denaturation of DNA, primer annealing and complementary chain synthesis

hreshold cycle value (Ct) is a cycle number at which the fluorescence generated within a reaction crosses the threshold