For Research Use Only. Not for use in diagnostic procedures.

Overview
KAPA Library Quantification Kits contain all the reagents needed for the accurate, reliable and reproducible qPCR-based quantification of next-generation sequencing (NGS) libraries prepared for sequencing on Illumina platforms. Kits include KAPA SYBR FAST qPCR Master Mix (formulated with different passive reference dyes for different qPCR instruments), a platform-specific library quantification primer premix, and a pre-diluted set of DNA standards. Refer to the Instrument Compatibility Chart for guidance on compatible platforms.

Benefits of KAPA Library Quantification Kits
Reliable and sensitive quantification of all sequencing-competent library molecules
Accurate and reproducible quantitation across a wide range of library types, concentrations, fragment length distributions and GC content
Accurate, equimolar pooling for multiplexed sequencing
Flexibility to support manual and automated high-throughput pipelines as well as PCR-free workflows

What makes KAPA Library Quantification Kits highly efficient for library quantification?
KAPA Library Quantification Kits contain KAPA SYBR FAST DNA Polymerase, which was engineered through our directed evolution technology to amplify diverse DNA fragments with similar efficiency. This is important for the amplification of heterogeneous populations such as NGS libraries. Library quantification kits containing wild-type DNA polymerases only count “easy” library molecules.